الفصل الرابع: التقنيات الأساسية لأشهر الصبغات الخاصة في الباثولوجي من صفحة 26
Chapter 4: The basics of some common required special stains
Objectives
1- Identify different methods used to detect carbohydrates and mucopolysaccharides (PAS, Microwave alcian blue, microwave alcian blue with hyaluronidase digestion and Modified Putchler Congo Red Amyloid Method with/without sodium permanganate oxidation)
2- Describe different methods to detect connective tissue (Masson‘s Trichrome stain and Verhoeff‘s elastic stain)
3- Identify different methods to detect cytoplasmic and nuclear elements (Microwave orcein method for hepatitis b surface antigen and Toluidine blue method for mast cells)
4- Describe different methods to detect pigments and metals (Microwave Rhodanine copper method and Perl‘s method for ferric iron).
5- Identify different methods to detect nerve cells and fibers (Thioflavin S method for amyloid in neurofibrillary plaques)

Type of special stain
Special stains in histopathology pdf
Special stain in histopathology
Staining in histopathology
Routine stains in histopathology
Tissue staining
Special stain in histopathology ppt
Glycogen stain

Course Description
This course aims at equip students with knowledge and skills about applied histopathology to enable students training on the different methods used in histopathology laboratories for the preparation and staining of different samples. Core Knowledge By the end of this course, students should be able to:
• Identify how to handle different tissue samples in tissue analysis laboratories
• Explain the methods of chemical preparation of different types of tissues, fluids and smears.
• Describe the method of routine staining of different samples on glass slides.
• Describe the methods of different special stains of samples on glass slides.
• Identify the steps of immunohistochemistry.
• Explain the preparation and staining of different samples in scanning electron microscope laboratory.
• Recognize the preparation artifacts and limitations and how to repair them. Core Skills By the end of this course, students should be able to:
• Apply different ways of preparation of tissues, fluids and smears.
• Demonstrate the routine staining of sample glass slides. Demonstrate the different special staining techniques of sample glass slides.
• Apply the immunohistochemistry techniques.
• Demonstrate the preparation and staining of different samples in scanning electron microscope laboratory.
• Apply the repair of different preparation artifacts.
• Use different senses to master the work
مقرر باثولوجيا الأنسجة التطبيقية
https://drive.google.com/file/d/1ho0gl0shSjp5CS-rtjNc-5vX9mr-Fq_3/view?usp=sharing
شرح الأستاذ الدكتور/ إيهاب حافظ
استشاري وأستاذ الباثولوجي بمعهد تيودور بلهارس والمعهد الفني الصحي بالقاهرة
شعبة المختبرات الطبية الفرقة الثانية الترم الثاني
المعاهد الفنية الصحية