الفصل الثالث: التقنيات الأساسية للصبغات المعتادة من صفحة 15
Chapter 3: The basics of routine staining techniques
Objectives
1-Identify types of routine staining techniques and advantages.
2-Identify different reagents uses and troubleshooting.
3-Explain the Rotation/Replacement of Solutions Guideline.
4-Describe General comments in preparing/using solutions.
Special stain in histopathology
Type of special stain
Staining in histopathology
Hematoxylin and eosin staining procedure
Special stains in histopathology pdf
Tissue staining
H&E stain
Types of staining
Course Description
This course aims at equip students with knowledge and skills about applied histopathology to enable students training on the different methods used in histopathology laboratories for the preparation and staining of different samples. Core Knowledge By the end of this course, students should be able to:
• Identify how to handle different tissue samples in tissue analysis laboratories
• Explain the methods of chemical preparation of different types of tissues, fluids and smears.
• Describe the method of routine staining of different samples on glass slides.
• Describe the methods of different special stains of samples on glass slides.
• Identify the steps of immunohistochemistry.
• Explain the preparation and staining of different samples in scanning electron microscope laboratory.
• Recognize the preparation artifacts and limitations and how to repair them. Core Skills By the end of this course, students should be able to:
• Apply different ways of preparation of tissues, fluids and smears.
• Demonstrate the routine staining of sample glass slides. Demonstrate the different special staining techniques of sample glass slides.
• Apply the immunohistochemistry techniques.
• Demonstrate the preparation and staining of different samples in scanning electron microscope laboratory.
• Apply the repair of different preparation artifacts.
• Use different senses to master the work
مقرر باثولوجيا الأنسجة التطبيقية
https://drive.google.com/file/d/1ho0gl0shSjp5CS-rtjNc-5vX9mr-Fq_3/view?usp=sharing
شرح الأستاذ الدكتور/ إيهاب حافظ
استشاري وأستاذ الباثولوجي بمعهد تيودور بلهارس والمعهد الفني الصحي بالقاهرة
شعبة المختبرات الطبية الفرقة الثانية الترم الثاني
المعاهد الفنية الصحية